MIDAS

MiDAS will deliver a self-contained, portable instrument to produce DNA database compatible results from crime samples in 2 hours. Automated processes for DNA analysis of large numbers of samples are now used in many of the EU member states; however the most efficient of these high throughput systems can take 3-5 days to produce DNA database-compliant profiles. In high profile crime and terrorism cases it is crucial to access DNA results promptly to provide intelligence to investigators.

Currently a small number of samples can be processed in less than 24 h but the method is labour intensive and expensive. MiDAS proposes a real-time, simple-to-operate and cost effective alternative. MiDAS will build on the current expertise of the consortium to deliver a robust, reliable instrument adaptable to the needs of multiple EU agencies. The design will allow processing of small numbers of samples at the scene of a crime, in an office environment or in the laboratory. Samples will be directly introduced to the device; no further manual involvement will be required.

DNA will be extracted using magnetic bead technology and transferred to reaction chambers for quantification and amplification which will be controlled to normalise the DNA profile. The profile will be visualised following analysis through on-cartridge DNA separation and laser induced fluorescence based detection. Automated data analysis using expert systems will facilitate the timely delivery of a profile. The requirements for implementation of the instrument into the forensic process will be determined, including an evaluation of relevant legislative and data protection requirements.

Successful forensic validation of the instrument and subsequent validation of the full forensic process designed around the instrument will allow implementation of MiDAS to the forensic community within the partner EU member states, enabling the delivery of early DNA intelligence to law enforcement and security agencies.