A systems biology approach to dissect cilia function and its disruption in human genetic disease

dysfunction caused by mutations in inherited ciliopathies, applying a systems biology approach.

BACKGROUND: Cilia are microtubule-based, centriole-derived projections from the cell surface. They transduce extracellular signals and regulate key processes in which signals of the extracellular environment are translated into a cellular response, such as cell cycle control, Wnt signalling, Shh signalling and planar cell polarity. Disruption of cilium-based processes by mutations can cause very severe disorders. Many of these ciliopathies have overlapping phenotypes. There is evidence, that ciliary proteins are organized in cell/context specific complexes and/or in shared regulatory circuits in cilia of affected tissues. Yet, knowledge of the composition, wiring, dynamics and associated signalling pathways of the corresponding molecular building blocks and associated protein networks remains very limited.

APPROACH: We propose here that ciliopathies can be considered systemically as specific perturbations in a versatile dynamically regulated multifunctional molecular machine. Mainly based on the comprehensive description of the ciliary interactome, quantitative functional assays as well as human genetic data derived from ciliopathy patients, we will generate a comprehensive stream of content-rich quantitative data towards systemic analysis of ciliar function. These data will be used to generate and validate discrete models that describe functional modules and regulatory circuits in the ciliome as well as predicting biological context specific features of cilia as well as perturbations leading to ciliopathies.
This will enable us to
1) understand the systemic features of discrete ciliary functions,
2) scrutinize the molecular disease mechanisms of different overlapping ciliopathies, and
3) develop therapeutic strategies towards improved treatment.

Short name and number: 
SYSCILIA, 241955
Name of US Partner: 
DUKE UNIVERSITY
Contact: 

DUKE UNIVERSITY

Administrative contact: Nicholas KATSANIS (Professor)
BLACKWELL ST SUITE 920 324, DURHAM, UNITED STATES
Tel: +1-9196848994
Fax: +1-9196848090

http://duke.edu/

Coordinator:

STICHTING KATHOLIEKE UNIVERSITEIT

Administrative contact: Maarten VAN LANGEN (Mr)
Comeniuslaan 4, 9102, NIJMEGEN, NEDERLAND
Tel: +31-243-619791
Fax: +31-243-540529

 

 

Participating Countries: 
France
Germany
Ireland
Italy
The Netherlands
United Kingdom
United States
Area: 

Health

Category: 

FP7 Project with U.S. partner